Human P1NP Plasma & Culture Media OneStep ELISA Kit

Catalog Number: S0C3230 Reactivity: Hu Conjugation: Brand: Starter

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$120 USD

Size: 1x24T 1x48T 1x96T 5x96T 10x96T

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Product Details

Product Specification

Antigen	Human P1NP
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Hu
Purification	Protein A
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay: 3.1%； Inter-assay: 3.9%
Sample type	EDTA Plasma, Cell Culture Supernatant
Assay type	Sandwich (quantitative)
Sensitivity	18.75 pg/mL
Range	54.7 pg/mL – 3500 pg/mL
Recovery	EDTA Plasma: 104% Cell Culture Supernatant: 95%
Assay time	60 minutes
Species reactivity	Hu

Background

Human P1NP (N-terminal propeptide of type I procollagen) is a 35-kDa peptide released during the extracellular processing of type I procollagen by osteoblasts. Structurally, it exists in circulation as both an intact trimeric form and smaller monomeric fragments, with the intact trimeric molecule being cleared by scavenger receptors in liver endothelial cells. Functionally, P1NP reflects the rate of type I collagen synthesis, making it the reference biomarker for bone formation. Clinically, serum P1NP is widely used to monitor osteoporosis treatment response (both antiresorptive and anabolic agents), assess Paget's disease, and evaluate bone turnover in conditions such as osteogenesis imperfecta and bone metastases.

Picture

ELISA

Standard Curve
Example of Human P1NP standard curve in Assay Diluent X1. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.

Spike Recovery
The recovery of Human P1NP was assessed by spiking a standard into plasma. Serial dilutions of the neat biological sample were prepared, and the percent recovery was calculated for each dilution relative to the expected concentration.

Spike Recovery
The recovery of Human P1NP was evaluated in medium spiked with concentrations spanning the entire assay range.

Spike-and-dilution Linearity
To evaluate assay linearity, samples were spiked with high levels of Human P1NP in matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE

HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.