Schematic diagram of Human NK Cell Panel Kit
Human NK Cell Panel Kit
Human NK Cell Panel Kit
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$420 USD
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Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human peripheral blood cells |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This three‑color panel is designed for detecting natural killer (NK) cells in human peripheral blood or other biological samples. CD3 was included as a T‑cell marker to exclude T lymphocytes. CD56 is a marker often used to detect NK cells, and CD16 is included to further characterize NK cell subsets; the combination of CD16 and CD56 expression can identify the major NK cell population as early NK cells，Mature NK cells and Terminal NK cells. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
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FC
Human peripheral blood cells were stained using the Human NK Cell Panel consisting of anti-human CD3 Alexa Fluor® 647, anti-human CD16 Alexa Fluor® 488 and anti-human CD56 PE conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the human peripheral blood cells excluding cellular debris.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the human peripheral blood cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 Alexa Fluor® 647-A vs SSC-A dot plot and apply the CD45+ gate.
4. Create a CD16 Alexa Fluor® 488-A vs CD56 PE-A dot plot and apply the CD3- gate. Create gates to encompass CD16+ CD56+ cells (NK cells).
