Standard curve
Example of Human hemoglobin standard curve in Assay Diluent DC4.
Human & Monkey Hemoglobin Serum & Plasma OneStep ELISA Kit
Human & Monkey Hemoglobin Serum & Plasma OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | Hemoglobin |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu, Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 1.9% Inter-assay: 2.7% |
| Sample type | Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.353 ng/mL |
| Range | 8.78 ng/mL – 100 ng/mL |
| Recovery | Human Serum: 108% Heparin Plasma: 81% EDTA Plasma: 93% |
| Assay time | 60 minutes |
| Species reactivity | Hu; Mk |
| CROSS REACTIVITY | Rt |
Background
Human hemoglobin is a complex metalloprotein found in red blood cells that is essential for transporting oxygen from the lungs to body tissues and facilitating the return transport of carbon dioxide. Structurally, it is a tetramer consisting of two alpha and two beta polypeptide chains in adults (HbA), each bound to a heme group containing a ferrous iron atom that can reversibly bind one oxygen molecule, allowing a single hemoglobin molecule to carry up to four oxygen molecules. Functionally, hemoglobin exhibits cooperative binding, where the binding of one oxygen molecule increases the affinity for the next, resulting in a sigmoidal oxygen dissociation curve modulated by factors such as pH (the Bohr effect), carbon dioxide, and 2,3-bisphosphoglycerate (2,3-BPG) to adapt oxygen release to tissue metabolic needs. Mutations in the globin genes can lead to disorders like sickle cell disease and thalassemias, making the understanding of hemoglobin’s structure and function fundamental to both physiology and clinical medicine.
Picture
Picture
ELISA
Linearity
The concentrations of Human hemoglobin were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human serum was 10fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 535.69 ng/mL in human serum.
#2 sample is undiluted samples are as follows: Human plasma-EDTA was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 256.82 ng/mL in Human plasma-EDTA.
#3 sample is undiluted samples are as follows: Human plasma-EDTA-donor#1 was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 53.44 ng/mL in Human plasma-EDTA donor#1.
#4 sample is undiluted samples are as follows: Human plasma-EDTA-donor#2 was 3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 390.37 ng/mL in Human plasma-EDTA-donor#2.
#5 sample is undiluted samples are as follows: Human plasma -Heparin was 15fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1810.71 ng/mL in Human plasma -Heparin.
Linearity
The concentrations of Human hemoglobin were measured and interpolated from the target standard curves and corrected for sample dilution.
#6 sample is undiluted samples are as follows: Female monkey serum was 10fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 465.74 ng/mL in female monkey serum.
Linearity
The concentrations of Human hemoglobin were measured and interpolated from the target standard curves and corrected for sample dilution.
#7 sample is undiluted samples are as follows: SD rat serum was 10fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 338.19 ng/mL in SD rat serum.
To assess the linearity of the assay, the sample was spiked with high concentrations of Human hemoglobin in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, the sample was spiked with high concentrations of Human hemoglobin in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram
