Standard curve
Example of Mouse Human CXCL10 curve in Assay Diluent #11.
Human & Monkey CXCL10(serum/plasma) OneStep ELISA Kit
Human & Monkey CXCL10(serum/plasma) OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | CXCL10 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu, Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.7%； Inter-assay: 8.1% |
| Sample type | Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.222 pg/mL |
| Range | 2.34 pg/mL – 150 pg/mL |
| Recovery | Serum: 111% Plasma: 100% |
| Assay time | 60 minutes |
| Species reactivity | Hu; Mk |
Background
CXCL10 is a small signaling protein classified as a chemokine. It is primarily produced by cells like macrophages and fibroblasts in response to interferon-gamma. Its main function is to act as a chemoattractant, guiding immune cells to sites of inflammation or infection. Specifically, CXCL10 binds to a receptor called CXCR3, which is found on the surface of activated T cells and natural killer (NK) cells. This interaction is crucial for mounting an effective immune response against viruses and intracellular pathogens. Consequently, CXCL10 plays a significant role in autoimmune diseases, chronic inflammation, and anti-tumor immunity. Its expression is often used as a biomarker for Th1-type immune responses. In summary, CXCL10 is a key mediator that recruits specific immune cells to coordinate the body's defense mechanisms.
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ELISA
Linearity
The concentrations of Human CXCL10 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human plasma was 2 fold dilution (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 140.24 pg/mL in stimulated human plasma.
Linearity
The concentrations of Human CXCL10 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human serum was 2 fold dilution (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 152.57 pg/mL in stimulated human serum.
Linearity
The concentrations of Human CXCL10 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: monkey serum was 16 fold dilution (6.25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 310.52 pg/mL in stimulated monkey serum.
Specificity
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