Human PBMCs were stained using the Human MLR Panel Kit consisting of anti-human CD3 APC-Cy7, anti-human HLA-DR PerCP-Cy5.5, anti-human CD11c PE, anti-human CD86 APC and anti-human CD80 PE-Cy7 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD11c PE-A vs HLA-DR PerCP-Cy5.5-A dot and apply the CD3- gate. Create gates to encompass CD11c+ HLA-DR+ cells (Conventional dendritic cells)
4. Create a CD80 PE-Cy7-A or CD86 APC-A vs SSC -A dot plot and apply the CD11c+ HLA-DR+ gate. Positivity for CD80 and CD86 is indicative of mature and activated DC populations.
Human MLR Panel Kit
Human MLR Panel Kit
Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This five‑color panel is designed for evaluating dendritic cell (DC) maturation and activation status in human peripheral blood or other biological samples. CD3 was included as a T‑cell marker to gate out T lymphocytes, ensuring that the analysis focuses on the CD3‑negative population. HLA‑DR and CD11c were included to identify conventional dendritic cells (cDCs) . Finally, the expression of CD80 and CD86 on the gated cDC population is indicative of DC maturation and activation; positivity for either marker reflects an activated state, and combined expression can further distinguish mature DC subsets. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest, for more detailed functional characterization.
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