Standard curve
Example of Human lysozyme standard curve in Assay Diluent #1.
Human Lysozyme OneStep ELISA kits
Human Lysozyme OneStep ELISA kits
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Product Details
Product Details
Product Specification
| Antigen | Lysozyme |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.3%； Inter-assay: 6.4% |
| Sample type | Cell culture supernatant, Serum,Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.167 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Recovery | Cell culture supernatant: 87% Serum: 86% Plasma: 102% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
Human lysozyme (LZM/LYZ), a 14.4-kDa bacteriolytic enzyme encoded by the LYZ gene on chromosome 12, functions as a critical innate immune defender by hydrolyzing β-1,4-glycosidic bonds in peptidoglycan of bacterial cell walls. Primarily secreted by neutrophils, macrophages, and epithelial cells, it is abundantly present in secretions (tears, saliva) and biological fluids, with serum/plasma concentrations typically ranging from 1.5–3.0 μg/mL in healthy adults. Clinically, elevated serum levels (>5 μg/mL) serve as a biomarker for bacterial infections (e.g., tuberculosis, meningitis), monocytic leukemia (due to malignant cell release), and renal tubular dysfunction (reduced reabsorption), while decreased levels correlate with immunosuppression. Detection relies on high-sensitivity ELISA (limit: ~0.5 ng/mL), though sample handling requires rigorous centrifugation and -80°C storage to prevent degradation. Its dual role as an antimicrobial agent and disease indicator underscores its significance in both physiological immunity and pathological diagnostics.
Picture
Picture
ELISA
Linearity
The concentrations of Human lysozyme were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: Human serum was 2X10^3 fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 724.69 ng/mL in human serum.
Linearity
The sample is undiluted samples are as follows: Human plasma was 2X10^3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 594.69 ng/mL in human plasma.
To assess the linearity of the assay,two samples were spiked with high concentrations of Human Lysozyme in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay,the sample was spiked with high concentrations of Human Lysozyme in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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