WB result of Human lgG3 F(c) rabbit mAb
Primary antibody: Human lgG3 F(c) (HRP Conjugate) rabbit mAb at 1/1000 dilution
Lane 1: IgG1 FC recombinant protein lysate 10 ng
Lane 2: IgG2 FC recombinant protein lysate 10 ng
Lane 3: IgG3 FC recombinant protein lysate 10 ng
Lane 4: IgG4 FC recombinant protein lysate 10 ng
Negative control: IgG1 FC recombinant protein lysate; IgG2 FC recombinant protein lysate; IgG4 FC recombinant protein lysate
Predicted MW: 33kDa
Observed MW: 38kDa
Human lgG3 F(c) Recombinant Rabbit mAb (HRP Conjugate) (S-459-75)
Human lgG3 F(c) Recombinant Rabbit mAb (HRP Conjugate) (S-459-75)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P01860 |
| Clone Number | S-459-75 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | HRP |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
Background
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4.
Picture
Picture
Western Blot
