Human IL-7 ELISA Kit
Human IL-7 ELISA Kit
Product Details
Product Details
Product Specification
| Theory | The kit is a double-antibody sandwich ELISA method, which is precoated with RNase Inhibitor to capture antibody. After adding the sample, the sample is captured to form antibody-antigen complex, and then HRP-labeled RNase Inhibitor is added to detect antibody. An antibody-antigen-antibody "sandwich" complex was formed. Finally, TMB was added for color development, and the absorbance (OD) value was read at 450nm wavelength after the reaction was terminated. The RNase Inhibitor content in the sample was positively correlated with the OD value. |
| Description |
Detection Principle: This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti human IL-7 antibody was pre coated on a high affinity microplate. Add the standard, sample to be tested and biotinylated detection antibody into the wells of enzyme plate. After incubation, IL-7 in the sample combines with solid-phase antibody and detection antibody. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate TMB was added to avoid light for color development. The depth of the color response is directly proportional to the concentration of IL-7 in the sample. Stop solution was added to stop the reaction, and the absorbance value was measured at 450 nm wavelength (reference wavelength 570-630 nm) Detection Type: Double antibody sandwich method
Form: Pre coated 96 well plate
Test Sample Type: Cell supernatant, serum, plasma
Loading Amount: 100 μ L
Kit Components: a copy of pre coated 96 well plate, standard, IL-7 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions
Sensitivity: 0.22 pg/ml
Detection Range: 7.18-500pg/ml
Recovery Range: 80-115%
Storage Method: 2-8 ℃
Standard Curve:
Background: Interleukin 7 (IL-7)It is a hematopoietic growth factor secreted by bone marrow and thymic stromal cells. It can also be produced by keratinocytes, dendritic cells, |
