Standard curve
Example of Human IL-6 standard curve in Assay Diluent FE6.
Human IL-6 OneStep ELISA Kit (High Level)
Human IL-6 OneStep ELISA Kit (High Level)
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Product Details
Product Details
Product Specification
| Antigen | IL-6 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay: 3.6%； Inter-assay: 7.2% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.466 pg/mL |
| Range | 2.1 pg/mL – 136 pg/mL |
| Recovery | Cell culture media: 88% Serum: 109% Plasma: 88% |
| Assay time | 60 minutes |
| Species reactivity | Hu, Mk |
| NIBSC convert | The dose response curve of the International Standard (21/308) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (21/308) units, use the following equation: NIBSC (21/308) approximate value (IU/mL) = 0.142 x OneStep Human IL-6 value (pg/mL). |
Background
Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.
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ELISA
Linearity
The concentrations of Human IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 6 days (0.1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 352.1 ng/mL in stimulated human PBMC supernatant.
Linearity
The concentrations of Human IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution. Sample is undiluted samples are as follows: human PBMC cells culture for 6 days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 113.7 pg/mL in unstimulated human PBMC supernatant.
Linearity
The concentrations of IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: monkey PBMC cells stimulated with 10ug/ml PHA for 5days (2%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3573.76 pg/mL in stimulated monkey PBMC supernatant.
#2 sample is undiluted samples are as follows: monkey PBMC cells culture for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 34.63 pg/mL in unstimulated monkey PBMC supernatant.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human IL-6 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Protocol Diagram
