Human IL-5 OneStep ELISA Kit

Catalog Number: S0C3009-HL Reactivity: Hu Conjugation: Brand: Starter

Price:

$500 USD

Size: 1x96T 5x96T 10x96T

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Product Details

Product Specification

Antigen	IL-5
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Hu
Purification	Protein A
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

Kit

Precision	Intra-assay: 4.7%； Inter-assay: 6.4%
Sample type	Cell culture supernatant; Serum; Plasma-Citrate; Plasma-EDTA
Assay type	Sandwich (quantitative)
Sensitivity	1.1 pg/mL
Range	3.9 pg/mL – 250 pg/mL
Recovery	Cell culture supernatant: 97% Serum: 76% Plasma-Citrate: 100% Plasma-EDTA: 96%
Assay time	60 minutes
Species reactivity	Hu
NIBSC convert	The dose response curve of the International Standard (90/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (90/586) units, use the following equation: NIBSC (90/586) approximate value (IU/mL) = 0.01 x OneStep Human IL-5 value (pg/mL).

Background

IL-5 protein, also known as Interleukin-5, is a protein molecule produced by the immune system and belongs to the category of cytokines. It is primarily expressed by T lymphocytes and other immune cells, such as NK cells. IL-5 plays a crucial role in regulating and mobilizing the body's immune response. IL-5 protein is critical for the generation, proliferation, and activation of eosinophils and basophils. These cells play significant roles in allergic diseases (such as allergic asthma and rhinitis) and certain parasitic infections. Given these characteristics of IL-5 protein, it has potential applications in in vitro diagnostics. By measuring the levels of IL-5 protein in bodily fluids, it can assist in diagnosing diseases associated with eosinophils or basophils, such as eosinophilic asthma and allergic rhinitis. These diseases are often linked to excessive expression or dysfunctional IL-5 protein. Furthermore, the level of IL-5 protein can reflect the state of the immune system.

Picture

ELISA

Standard curve
Example of Human H-FABP standard curve in Assay Diluent DA4.

Linearity
The concentrations of Human IL-5 were measured and interpolated from the target standard curves and corrected for sample dilution.
The sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 178.95 pg/mL in stimulated human PBMC supernatant.

To assess the linearity of the assay, the sample was spiked with high concentrations of Human IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

To assess the linearity of the assay, two samples were spiked with high concentrations of Human IL-5 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.