Standard curve
Example of Human IL-4R standard curve in Assay Diluent #11DS.
Human IL-4R OneStep ELiSA Kit
Human IL-4R OneStep ELiSA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-4R |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.5%； Inter-assay: 6.1% |
| Sample type | Cell culture supernatant; Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.053 pg/mL |
| Range | 2.34 pg/mL – 150 pg/mL |
| Recovery | Cell culture supernatant: 91% Serum: 103% Plasma: 103% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Human IL-4R (Interleukin-4 Receptor) is a critical cell surface receptor involved in immune regulation and inflammatory responses, composed of the IL-4Rα chain (CD124) paired with either the common γ-chain (γc, CD132) or IL-13Rα1 to form Type I or Type II receptors. It binds cytokines IL-4 and IL-13, activating JAK-STAT signaling pathways that modulate immune cell functions, particularly in hematopoietic cells such as B cells, T cells, macrophages, and mast cells. IL-4R signaling promotes Th2 cell differentiation, IgE antibody class switching, and anti-inflammatory responses, playing a central role in allergic and immune-mediated diseases. Dysregulation of IL-4R is associated with conditions like asthma, atopic dermatitis, and autoimmune disorders. Due to its therapeutic potential, IL-4R has been successfully targeted by drugs such as dupilumab, offering effective treatment for allergic diseases. Ongoing research continues to explore IL-4R's broader roles in immunity and inflammation, paving the way for novel therapies targeting immune dysregulation.
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ELISA
Linearity
The concentrations of Human IL-4R were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human plasma (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 565.71 pg/mL in human plasma #1. The mean target concentration was determined to be 739.72 pg/mL in human plasma #2.
Linearity
The concentrations of Human IL-4R were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human serum (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 464.86 pg/mL in human serum #1. The mean target concentration was determined to be 472.25 pg/mL in human serum #2.
Demonstration of specificity in mice
The sample dilution factor was 4-fold.
The added human IL-4R was detected in mouse serum, with a recovery rate of 101%.
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Protocol Diagram
