Standard Curve
Example of Human IL-28A standard curve in Assay Diluent EA8. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.
Human IL-28A/IFN-λ2 OneStep ELISA Kit
Human IL-28A/IFN-λ2 OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IL-28A/IFN-λ2 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 7.3%； Inter-assay: 8.6% |
| Sample type | Serum; EDTA Plasma, Heparin Plasma, Citrate Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 77.8 pg/mL |
| Range | 1,844 pg/mL –21,000 pg/mL |
| Recovery | Serum: 106% EDTA Plasma: 112% Citrate plasma: 105% Heparin Plasma: 114% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
IL-28A/Interferon-lambda 2) is a type III interferon that is upregulated in response to viral infection. It acts primarily on epithelial cells through a receptor complex that contains IL-10 R beta and IL-28 R alpha/IFN-lambda R1. IL-28A promotes the Th1 polarization of dendritic cells in the airway (e.g. production of IL-12 p70 and IFN-gamma) and inhibits Th2 and Th17 mediated inflammation. In the liver, however, the IL-28A induced Th1 cytokine response contributes to inflammation in T cell mediated hepatitis. IL-28A additionally exhibits anti-tumor activity, in part by enhancing IL-12 dependent anti-tumor CTL responses. In contrast, it is upregulated in invasive bladder cancer where it promotes tumor cell migration.
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ELISA
Spike Recovery
The recovery of Human IL-28A was evaluated in activated samples spiked with concentrations spanning the entire assay range.
Spike-and-dilution Linearity
To evaluate assay linearity, three samples were spiked with high levels of Human IL-28A in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.
Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE
Cross-reactivity
The cross-reactivity between Human IL-28A and Mouse IL-28A was assessed by testing both proteins in the same assay system. Serial dilutions of each protein were measured, and the dose-response curves were compared. No significant cross-reactivity was observed when Human NT-pro BNP was tested in the Human IL-28A assay, as the signals remained within the background range of the assay.
HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.
Protocol Diagram
