Standard Curve
Example of Human IL-23 standard curve in Assay Diluent HA6 Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.
Human IL-23 Serum & Plasma OneStep ELISA Kit
Human IL-23 Serum & Plasma OneStep ELISA Kit
Price:
Regular price
$700 USD
Regular price
Sale price
$700 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | IL-23 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 1.8%； Inter-assay: 2.2% |
| Sample type | Serum; EDTA Plasma, Heparin Plasma, Citrate Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 30.19 pg/mL |
| Range | 78.1 pg/mL – 5000 pg/mL |
| Recovery | Serum: 107% EDTA Plasma: 110% Citrate plasma: 116% Heparin Plasma: 106% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Human interleukin-23 (IL-23) is a pro-inflammatory, heterodimeric cytokine belonging to the IL-12 family. It is composed of two subunits: a unique p19 subunit (IL-23A) and a p40 subunit (IL-12B), the latter being shared with IL-12. This 53.5–59 kDa glycoprotein is predominantly secreted by activated antigen-presenting cells, such as dendritic cells and macrophages. IL-23 plays a central role in adaptive immunity by driving the expansion, stabilization, and survival of T helper 17 (Th17) cells, which are critical for host defense against extracellular pathogens at mucosal barriers. It exerts its biological effects by signaling through a heterodimeric receptor complex composed of its specific IL-23R and the shared IL-12Rβ1 subunit, activating the JAK-STAT signaling pathway. Functionally, IL-23 is a key mediator in autoimmune and chronic inflammatory diseases, including psoriasis, psoriatic arthritis, Crohn's disease, and rheumatoid arthritis, making it a prominent therapeutic target.
Picture
Picture
ELISA
Spike Recovery
The recovery of Human IL-23 was evaluated in activated samples spiked with concentrations spanning the entire assay range.
Spike-and-dilution Linearity
To evaluate assay linearity, three samples were spiked with high levels of Mouse NT-Pro BNP in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.
Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE
Cross‑reactivity
The cross‑reactivity between Human IL-23 and Mouse IL-23 was assessed by testing both proteins in the same assay system. Serial dilutions of each protein were measured, and the dose‑response curves were compared. No significant cross‑reactivity was observed when Mouse IL-23 was tested in the Human IL-23 assay, as the signals remained within the background range of the assay.
HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.
Protocol Diagram
