Human IL-23 culture media OneStep ELISA Kit

Catalog Number: S0C3189 Reactivity: Hu Conjugation: Brand: Starter

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$700 USD

Size: 1x96T 5x96T 10x96T

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Product Details

Product Specification

Antigen	IL-23
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Hu
Purification	Protein A
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay:2.8%； Inter-assay: 5.2%
Sample type	Cell Culture Supernatant
Assay type	Sandwich (quantitative)
Sensitivity	19.98 pg/mL
Range	78.1 pg/mL – 5000 pg/mL
Recovery	Cell Culture Supernatant: 101%
Assay time	60 minutes
Species reactivity	Hu

Background

Human IL-23 (interleukin-23) is a heterodimeric cytokine composed of a p19 subunit and a shared p40 subunit, produced primarily by activated dendritic cells and macrophages. It is a critical driver of the Th17 cell response and promotes the production of pro-inflammatory cytokines such as IL-17A and IL-22. Unlike homeostatic interleukins, IL-23 has no significant role in basal immune surveillance and is upregulated rapidly under inflammatory conditions. Its secretion increases markedly in response to microbial stimuli, tissue injury, and autoimmune signals. In human cell culture models, elevated IL-23 levels in conditioned media serve as a reliable indicator of inflammatory activation and antigen-presenting cell function. Researchers commonly measure human IL-23 in culture supernatants to evaluate immune responses in conditions such as psoriasis, inflammatory bowel disease, and rheumatoid arthritis. Because it remains relatively stable in culture media under controlled conditions, IL-23 is a practical and sensitive biomarker for preclinical immunology studies. Thus, it plays a valuable role in assessing the efficacy of biologics and small-molecule inhibitors in human inflammatory disease models.

Picture

ELISA

Standard Curve
Example of Human IL-23 standard curve in Assay Diluent HA8. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.

Spike Recovery
The recovery of Human IL-23 was evaluated in activated samples spiked with concentrations spanning the entire assay range.

Dilution Linearity
The concentrations of Human IL-23 were measured and interpolated from the target standard curves and corrected for sample dilution.
The leftmost bar in the graph shows biological samples diluted in Assay Diluent to 50% concentration. "undiluted" was defined as 50% biological sample. The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 3042.45 pg/mL in THP-1 cell culture supernatant stimulated with 100 ng/mL LPS for 6 hours.

Spike-and-dilution Linearity
To evaluate assay linearity, three samples were spiked with high levels of Human IL-23 in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE

HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.