Human IL-22 Single Plex Assay (适用于Luminex)

Catalog Number: S0X3058 Reactivity: Human Conjugation: Brand: Starter

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$585 USD

Size: 1x96T

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Product Details

Product Specification

Antigen	IL-22
Reactivity	Human
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay: ＜10%； Inter-assay: ＜15%
Sample type	Serum; Plasma; Cell culture supernatant
Assay type	Sandwich (quantitative)
Sensitivity	＜5 pg/mL
Range	15.65pg/mL-125000pg/mL
Recovery	Plasma: 80%-120% Serum: 80%-120% Cell culture supernatant: 80%-120%
Assay time	70 minutes
Species reactivity	Human

Background

Interleukin-22(IL-22) is a member of the IL10 family of cytokines that mediate cellular inflammatory responses and functions in antimicrobial defense at mucosal surfaces and in tissue repair. This protein also has pro-inflammatory properties and plays a role in in the pathogenesis of several intestinal diseases. IL-22 has a role in the remodeling of the uterine tissue in the inflammatory environment by regulating epithelial-mesenchymal transition markers called E- and N-cadherin. Therefore, IL-22 contributes to the proper regeneration of endometrial layers after inflammation-triggered abortion. Thus, it might have a practical significance to be utilized as a treatment option postpartum (enhanced regeneration function) and in secondary infertility caused by inflammation (enhanced barrier/protector function). IL-22 is a crucial cytokine that regulates host immunity in infectious diseases, including COVID-19 (disease caused by SARS-CoV-2).

Picture

Luminex

Standard curve
Example of IL-22 standard curve in Assay Diluent .

Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with CD3、PMA、PHA、LPS、Poly(I:C), and the average concentration of IL-22 in the supernatant was measured to be 16.23 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-22 in the supernatant was measured to be 13.47 pg/mL.

Recovery
Within the detection range, the combined cytokine proteins were added to serum,plasma,cell supernatant at high、low concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.