Standard curve
Example of Human IL-2 standard curve in Assay Diluent #11E4.
Human IL-2 OneStep ELISA Kit (High Level)
Human IL-2 OneStep ELISA Kit (High Level)
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Product Details
Product Details
Product Specification
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Kit
| Precision | Intra-assay:4.4%； Inter-assay: 6.2% |
| Sample type | Serum,Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.990 pg/ml |
| Range | 3.13 pg/mL – 200 pg/mL |
| Recovery | Serum: 111% Plasma: 92% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Picture
Picture
ELISA
Linearity
The concentrations of Human IL-2 were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: Jurkat T cells stimulated with 10 ng/ml PMA and 150 ng/ml A23187 for 24 h (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 4362.3 pg/mL in stimulated Jurkat supernatant.
To assess the linearity of the assay, two samples were spiked with high concentrations of Human IL-2 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Protocol Diagram
