Human IL-12p70 High Sensitivity ELISA Kit
Human IL-12p70 High Sensitivity ELISA Kit
Product Details
Product Details
Product Specification
| Species Reactivity | Human |
| Synonym | CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL12 p70, IL-12 p70, IL-12 subunit p35, IL-12, subunit p35, interleukin 12, p35, interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35), interleukin-12 alpha chain, interleukin-12 subunit alpha, natural killer cell stimulatory factor 1, 35 kD subunit, NF cell stimulatory factor chain 1, NK cell stimulatory factor chain 1, NKSF1, p35 |
| Description |
Detection principle: This kit uses double antibody sandwich ELISA Technologies. Specificity anti- Human IL-12 capture antibody Pre-coated on high affinity enzyme plates. The standard substance, the sample to be measured and the biotin-labeled detection antibody are sequentially added into the wells of the enzyme label plate, thoroughly shaken and mixed, and then placed at room temperature for time 2 Hours of incubation process, present in the sample IL-12 Binds to solid phase antibodies and detection antibodies. After sufficient washing to remove free and unbound components, horseradish peroxidase-labeled streptavidin was added (Streptavidin-HRP ， SA-HRP) 。 After washing, the signal enhancer was added for incubation, and after washing to remove unbound material, it was added again SA-HRP 。 After washing again, add TMB The chromogenic substrate is incubated at room temperature in the dark from light to develop color. The depth of color reaction and the sample IL-12 The concentration of is positively correlated. Add a stop solution to stop the reaction, and use a microplate reader to 450 nm Detection wavelength ( Correction wavelength 570-630 nm) The absorbance values were determined under conditions.
Test Type: Double antibody sandwich method
Form: Pre-coating 96 Orifice plate
Test Sample Type: Cell supernatant, serum, plasma
Sample Load: 100 μl
Kit Components: Pre-coating 96 Well plates, standards, IL-12p70 Test antibody, standard dilution, test buffer, signal enhancer, signal enhancer dilution, TMB A chromogenic substrate, a wash liquid, a stop liquid, SA-HRP , a copy of the sealing film and instructions.
Sensitivity: 0.04 pg/mL
Detection range: 0.39-25 pg/mL
Recovery Range: 91-104%
Save Method: 2-8℃
Standard graph:
Background: Interleukin 12(IL-12) Composed of dendritic cells, macrophages, neutrophils and human B Lymphoblastic cell line (NC-37) Arises naturally in response to antigenic stimulation. IL-12 By 4 1 а Helix composed, is a composed of 2 Species independent gene ---IL-12A(p35) And IL-12B(p40) Encoding the formed heterodimeric cytokine. Biologically active heterodimers can be formed after protein synthesis (p70) And homodimers p40 。 IL-12 Participate in initial T Cells to Th1 Process of cell differentiation, as T Cell stimulating factor, which stimulates T The growth of cells and their functions. IL-12 Has anti-angiogenic activity, and is effective against natural killer cells and T Lymphocyte activity plays an important role. Similarly, IL-12 Associated with autoimmunity. |
