Standard curve
Example of IL-10 standard curve in Assay Diluent .
Human IL-10 Single Plex Kit (Flow Cytometry Bead Assay)
Human IL-10 Single Plex Kit (Flow Cytometry Bead Assay)
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Product Details
Product Details
Product Specification
| Antigen | IL-10 |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 3%； Inter-assay: 5% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 1.41 pg/mL |
| Range | 5.13 pg/mL-5250 pg/mL |
| Recovery | Plasma: 107% Serum: 105% Cell culture supernatant: 114% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In humans, IL-10 is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type-II T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 can be produced by monocytes upon PD-1 triggering in these cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.
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CBA
Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA for 24h, and the average concentration of IL-10 in the supernatant was measured to be 153.07 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-10 in the supernatant was measured to be 11.72 pg/mL.
Linearity
The high-concentration human IL-10 protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of human IL-10 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.
Protocol Diagram
