Human IgM Single Plex Kit (Flow Cytometry Bead Assay)

Catalog Number: S0Q3026 Reactivity: Human Conjugation: Brand: Starter

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$500 USD

Size: 1x96T

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Product Details

Product Specification

Antigen	Human IgM
Reactivity	Human
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay:； Inter-assay:
Sample type	Serum; Plasma-EDTA; Plasma-Heparin; Plasma-Citrate; Cell Culture Supernatant
Assay type	Sandwich (quantitative)
Sensitivity	0.14 ng/mL
Range	0.37 ng/mL-270 ng/mL
Recovery	Serum: 105.12% Plasma-EDTA:98.93% Plasma-Heparin:91.85% Plasma-Citrate:96.48% Cell culture supernatant: 107.64%
Assay time	70 minutes
Species reactivity	Human

Background

Immunoglobulin M (IgM) is the largest of several isotypes of antibodies (also known as immunoglobulin) that are produced by vertebrates. IgM is the first antibody to appear in the response to initial exposure to an antigen; causing it to also be called an acute phase antibody. In humans and other mammals that have been studied, plasma blasts in the spleen are the main source of specific IgM production. IgM is the first immunoglobulin expressed in the human fetus (around 20 weeks) and phylogenetically the earliest antibody to develop. IgM antibodies appear early in the course of an infection and usually reappear, to a lesser extent, after further exposure. IgM antibodies do not pass across the human placenta (only isotype IgG). Demonstrating IgM antibodies in a patient's serum indicates recent infection, or in a neonate's serum indicates intrauterine infection.

Picture

CBA

Standard curve

Example of IgM standard curve in Assay Diluent .

Dilution Parallelism

Pre-diluted serum and plasma (EDTA, Heparin, Citrate) samples (1:20,000 in sample diluent) and cell culture supernatant (1:2 in sample diluent) (labeled as "Undiluted") were further serially diluted 2X, 4X, and 8X with sample diluent and assayed for Human IgM. Data shown on the y-axis represent the measured Human IgM concentrations multiplied by their respective serial dilution factors (ng/mL)

Accuracy and Spike Recovery

Serum and plasma (EDTA, Heparin, Citrate) matrices—pre-diluted at the minimum sample dilution (MSD = 1:20,000) in sample diluent and immunodepleted with target-specific magnetic beads to near the lower limit of linearity—along with cell culture supernatant (Supernatant) diluted at MSD = 1:2 in sample diluent, were spiked with high, medium, and low levels of Human IgM. The y-axis displays the calculated spike recovery percentages (Spike Recovery%), with values across all tested matrices ranging between 80% and 120%.

Sample Reactivity
Serum and plasma (EDTA, Heparin, Citrate) samples from human, mouse (CK), and rat (SD) were diluted at the minimum sample dilution (MSD = 1:20,000) and assayed for Human IgM (ng/mL). Measurable concentrations were detected in human samples, whereas all mouse and rat samples yielded values of 0 ng/mL.