WB result of Human IgG4 Recombinant Rabbit mAb
Primary antibody: Human IgG4 Recombinant mAb at 1/1000 dilution
Lane 1: human serum lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 50 kDa
Human IgG4 Recombinant Rabbit mAb (S-1140-53)
Human IgG4 Recombinant Rabbit mAb (S-1140-53)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Human IgG4 |
| Synonyms | Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P01861 |
| Clone Number | S-1140-53 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA, WB, IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20°C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:2000 | null |
| IF | 1:2000 | null |
Background
IgG4 is a subclass of immunoglobulin G (IgG), which comprises four main subclasses: IgG1, IgG2, IgG3, and IgG4. IgG4 primarily participates in immune regulation within the immune system and plays a significant role in certain inflammatory responses. For instance, it can form cross-links with other antibodies, preventing them from binding to antigens, thereby reducing the intensity of inflammatory reactions. Elevated IgG4 levels are often observed in autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and Sjögren's syndrome.
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Picture
Western Blot
WB result of Human IgG4 Recombinant Rabbit mAb
Primary antibody: Human IgG4 Recombinant mAb at 1/1000 dilution
Lane 1: IgG1 FC recombinant protein lysate 10 ng
Lane 2: IgG2 FC recombinant protein lysate 10 ng
Lane 3: IgG3 FC recombinant protein lysate 10 ng
Lane 4: IgG4 FC recombinant protein lysate 10 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 35 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Human IgG4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-Human IgG4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human tonsil. Anti-Human IgG4 antibody was used at 1/2000 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
