WB result of Human IgG F(c) rabbit mAb
Primary antibody: Human IgG F(c) (HRP Conjugate) rabbit mAb at 1/1000 dilution
Lane 1: IgG1 FC recombinant protein lysate 10 ng
Lane 2: IgG2 FC recombinant protein lysate 10 ng
Lane 3: IgG3 FC recombinant protein lysate 10 ng
Lane 4: IgG4 FC recombinant protein lysate 10 ng
Predicted MW: IgG1 FC, IgG2 FC, IgG4 FC: 27kDa; IgG3 FC: 33kDa
Observed MW: IgG1 FC, IgG2 FC, IgG4 FC: 33kDa; IgG3 FC: 38kDa
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | Human IgG |
Synonyms | Immunoglobulin heavy constant gamma |
Immunogen | Native protein |
Location | Secreted, Cell membrane |
Accession | P01859、 P01860、 P01861、 P01857 |
Clone Number | S-318-37 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | ELISA, WB |
Reactivity | Hu |
Purification | Protein A |
Concentration | 2 mg/ml |
Conjugation | HRP |
Physical Appearance | Liquid |
Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
application | dilution | species |
WB | 1:1000 | null |
Background
The intact antibody of human immunoglobulin (IgG) is composed of the fragment for antigen binding (Fab) and the crystallizable fragment (Fc) for binding of Fcγ receptors. Among the four subclasses of human IgG (IgG1, IgG2, IgG3, IgG4), which differ in their constant regions, particularly in their hinges and CH2 domains, IgG1 has the highest FcγR-binding affinity, followed by IgG3, IgG2, and IgG4.
Picture
Picture
Western Blot
