• Human IFA ELISA Kit
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Human IFA ELISA Kit

Human IFA ELISA Kit

Catalog Number: abs5510756 Application: ELISA Reactivity: Human Conjugation:
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$381 USD
$381 USD
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Product Details

Product Specification

protein IFA
Sensitivity 30 pg/mL
Species Reactivity Human
Theory This kit adopts the principle of sandwich method. The specific anti-human IFA antibody was coated in a 96-well microplate, and the human IFA standard or sample was added to the microwells, so that the human IFA protein in the standard or the human IFA protein in the sample was bound to the anti-human IFA antibody solid on the microplate, then biotinylated anti-human IFA antibody was added, the unbound biotinylated antibody was washed, HRP-labeled streptavidin was added, and TMB substrate was added to develop color. TMB is converted to blue under peroxidase catalysis and to final yellow under the action of acid. There was a positive correlation between the depth of color and the human IFA protein in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the sample concentration was calculated by drawing a standard curve.
Source Human
Synonym Human IFA(Intrinsic Factor Antibody) ELISA Kit;Gastric Intrinsic Factor; Gastric intrinsic factor (vitamin B synthesis); GIF; TCN3; TCM3
Detection Type Human IFA can be detected in a sample and does not cross-react with other related proteins
Composition

Chinese Name

96T

Preservation conditions

Enzyme labeled plate (detachable)

12 Strip x 8 Hole

4°C/-20°C

Lyophilized Standard

2

4°C/-20°C

Standard & Sample dilution

20 mL

4°C/-20°C

Concentrated biotinylated antibodies ( 100× ）

120 μL

4°C/-20°C

Biotinylated antibody dilution

12 mL

4°C/-20°C

concentrate HRP Enzyme conjugate ( 100× ）

120 μL

4°C/-20°C

Enzyme conjugate dilution

12 mL

4°C/-20°C

Concentrated wash ( 25× ）

20 mL

4°C/-20°C

Chromogenic substrate solution ( TMB ）

10 mL

4°C/-20°C( Protected from light)

Reaction stop solution

6 mL

4°C/-20°C

Sealing film

2

normal temperature

General Notes

1. Please make sure that all components are dissolved and mixed before using the kit. If the reconstituted standard is not used, please discard it.

2. Concentrate the biotinylated antibody. The volume of the concentrated enzyme conjugate is small and may be dispersed in various parts of the tube during transportation. Please centrifuge at 1000 × g for 1 minute before use, so that the liquid on the tube wall or bottle cap can be deposited to the bottom of the tube. Mix the solution by carefully pipetting 4-5 times with a pipette before taking. Standard, biotinylated antibody working solution and enzyme conjugate working solution should be prepared according to the required dosage, and the corresponding diluent should be used to prepare without confusion.

3. The concentrated washing liquid taken out of the refrigerator may have crystals, which is a normal phenomenon. The crystals can be completely dissolved in a water bath or incubator before preparing the washing liquid (the heating temperature should not exceed 40 °C). The wash liquid should be at room temperature when used.

4. Sample addition needs to be quick, and it is best to control each sample addition within 10 minutes. In order to ensure the accuracy of the experiment, it is recommended to use double holes. Maintaining a consistent sequence of addition from well to well when pipetting reagents will ensure the same incubation time for all wells.

5. During the washing process, the washing liquid remaining in the reaction hole should be patted dry on absorbent paper. Do not put the filter paper directly into the reaction hole to absorb water. Before reading, pay attention to removing the residual liquid and fingerprints at the bottom, so as not to affect the reading of the microplate reader.

6. The developer TMB should avoid direct exposure to strong light during storage and use. After adding the substrate, pay attention to the color change in the reaction well. If the gradient is obvious, please terminate the reaction in advance to avoid too dark color affecting the reading of the microplate reader.

7. The test tubes and reagents used during the experiment are disposable, and it is strictly forbidden to reuse them, otherwise the experimental results will be affected.

8. Please wear a laboratory coat and latex gloves for protection during the experiment, especially when testing blood or other body fluid samples, please follow the national biological laboratory safety protection regulations.

9. Kit components of different batch numbers cannot be mixed (except washing solution and reaction stop solution).

10. The enzyme labeling strip in the kit is a detachable plate, please use it in batches according to the experimental requirements.
Storage Temp. Unopened kit, stored at 4 °C, shelf life 6 months.
Test Range 78.13-5000 pg/mL