Human Granzyme B Single Plex Kit (Flow Cytometry Bead Assay)
Human Granzyme B Single Plex Kit (Flow Cytometry Bead Assay)
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$500 USD
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Product Details
Product Details
Product Specification
| Antigen | Granzyme B |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 3.19%； Inter-assay: 2.04% |
| Sample type | Serum; Plasma-EDTA; Plasma-Heparin; Plasma-Citrate; Cell Culture Supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 4.79 pg/mL |
| Range | 1.83 pg/mL-7500 pg/mL |
| Recovery | Serum: 90.40% Plasma-EDTA:117.31% Plasma-Heparin:73.68% Plasma-Citrate:107.28% Cell culture supernatant: 117.23% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
Granzyme B (GrB) is a serine protease pivotal in cytotoxic immune responses, mediating apoptosis and immune surveillance. Structurally, GrB is synthesized as a 32 kDa inactive pro-enzyme (zymogen) and activated through proteolytic cleavage. Its active form features a conserved catalytic triad (His57, Asp102, Ser195) essential for enzymatic activity. Post-translational modifications, including N-linked glycosylation, enhance its stability and regulate intracellular trafficking. GrB functions by binding to mannose-6-phosphate receptors (MPRs) for endosomal uptake into target cells, where it synergizes with perforin to induce cell death.
