Standard curve
Example of Granzyme B standard curve in Assay Diluent .
Human Granzyme B Single Plex Assay (适用于Luminex)
Human Granzyme B Single Plex Assay (适用于Luminex)
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Product Details
Product Details
Product Specification
| Antigen | Granzyme B |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: ＜10%； Inter-assay: ＜15% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | ＜8 pg/mL |
| Range | 5.47pg/mL-25000pg/mL |
| Recovery | Plasma: 80%-120% Serum: 80%-120% Cell culture supernatant: 80%-120% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
Granzyme B (GrB) is a serine protease pivotal in cytotoxic immune responses, mediating apoptosis and immune surveillance. Structurally, GrB is synthesized as a 32 kDa inactive pro-enzyme (zymogen) and activated through proteolytic cleavage. Its active form features a conserved catalytic triad (His57, Asp102, Ser195) essential for enzymatic activity. Post-translational modifications, including N-linked glycosylation, enhance its stability and regulate intracellular trafficking. GrB functions by binding to mannose-6-phosphate receptors (MPRs) for endosomal uptake into target cells, where it synergizes with perforin to induce cell death.
Picture
Picture
Luminex
Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with CD3、PMA、PHA、LPS、Poly(I:C), and the average concentration of Granzyme B in the supernatant was measured to be 136.23 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of Granzyme B in the supernatant was measured to be 18.47 pg/mL.
Recovery
Within the detection range, the combined cytokine proteins were added to serum,plasma,cell supernatant at high、low concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.
Protocol Diagram
