Standard curve
Example of GM-CSF standard curve in Assay Diluent .
Human GM-CSF Single Plex Kit (Flow Cytometry Bead Assay)
Human GM-CSF Single Plex Kit (Flow Cytometry Bead Assay)
Price:
Regular price
$335 USD
Regular price
Sale price
$335 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | GM-CSF |
| Reactivity | Human |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 2.44%； Inter-assay: 7.10% |
| Sample type | Serum; Plasma; Cell culture supernatant |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.64 pg/mL |
| Range | 2.33 - 10000 pg/mL |
| Recovery | 76.11% - 101.52% |
| Assay time | 70 minutes |
| Species reactivity | Human |
Background
GM-CSF is a hematopoietic growth factor secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. GM-CSF binds to receptor heterodimers consisting of a GM-CSF-specific alpha chain and the common beta chain that is shared by the high-affinity receptors for IL-3 and GM-CSF.
Picture
Picture
CBA
Recovery
Within the detection range, the human GM-CSF protein was added to different matrices at high, medium, low concentrations.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.
Quantifiction of human PBMC
Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA and 1μg/mL Iono for 24h, and the average concentration of GM-CSF in the supernatant was measured to be 1367.65 pg/mL.
Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of GM-CSF in the supernatant was measured to be 0.002 pg/mL.
Linearity
The high-concentration human GM-CSF protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively.
The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
Then, the concentration of human GM-CSF was determined and interpolated based on the target standard curve, and the sample dilution was corrected.
