Human ECM1 OneStep ELISA Kit

Catalog Number: S0C3243 Reactivity: Hu Conjugation: Brand: Starter

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$120 USD

Size: 1x24T 1x48T 1x96T 5x96T 10x96T

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Product Details

Product Specification

Antigen	ECM1
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Hu
Purification	Protein A
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay: 3.7%； Inter-assay: 4.9%
Sample type	Serum; EDTA Plasma, Heparin Plasma, Citrate Plasma; Cell Culture Supernatant
Assay type	Sandwich (quantitative)
Sensitivity	6.96 pg/mL
Range	31.3 pg/mL – 2000 pg/mL
Recovery	Serum: 105% EDTA Plasma: 100% Citrate plasma: 97% Heparin Plasma: 98% Cell Culture Supernatant: 88%
Assay time	60 minutes
Species reactivity	Hu

Background

Human ECM1 (extracellular matrix protein 1) is an 85-kDa secreted glycoprotein that adopts a serum albumin-like structural fold, consisting of an N-terminal alpha-helical domain and three serum albumin subdomain-like (SASDL) domains. These domains form four finger-like projections that enable the protein to bind a wide variety of ligands. Functioning as a "biological glue," ECM1 plays a critical role in maintaining tissue integrity, particularly in the skin, by interacting with structural proteins such as perlecan, laminin 332, and fibulin-3, and by inhibiting matrix metalloproteinase 9 (MMP9) activity. Clinically, loss-of-function mutations in ECM1 cause lipoid proteinosis, a rare autosomal recessive disorder characterized by skin and mucosal lesions, whereas overexpression of ECM1 is strongly associated with tumor progression, invasion, and metastasis in several cancers, including breast and lung cancer. Because of its dual role in tissue homeostasis and disease, ECM1 is considered both a potential therapeutic target and a diagnostic biomarker.

Picture

ELISA

Standard Curve
Example of Human ECM1 standard curve in Assay Diluent A1. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.

Spike Recovery
The recovery of Human ECM1 was assessed by spiking a standard into serum or plasma. Serial dilutions of the neat biological sample were prepared, and the percent recovery was calculated for each dilution relative to the expected concentration.

Spike Recovery
The recovery of Human ECM1 was evaluated in medium spiked with concentrations spanning the entire assay range.

Dilution linearity
The concentrations of Human ECM1 were measured and interpolated from the target standard curves and corrected for sample dilution.
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (0.067%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 3070.3 ng/mL in human serum.

The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (0.033%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 5686.7 ng/mL in human EDTA plasma.

The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (0.04%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 4469.8 ng/mL in human heparin plasma.

The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (0.125%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 1386.1 ng/mL in human citrate plasma.

Spike-and-dilution Linearity
To evaluate assay linearity, Cell culture media was spiked with high levels of Human ECM1 in matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE

HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.