Human PBMCs were stained using the Human DC Panel Kit consisting of anti-human HLA-DR PerCP-Cy5.5, anti-human CD11c PE, anti-human CD86 APC and anti-human CD80 PE-Cy7 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD11c PE-A vs HLA-DR PerCP-Cy5.5-A dot and apply the leukocyte population. Create gates to encompass CD11c+ HLA-DR+ cells (Conventional dendritic cells)
4. Create a CD80 PE-Cy7-A or CD86 APC-A vs SSC -A dot plot and apply the CD11c+ HLA-DR+ gate. Positivity for CD80 and CD86 is indicative of mature and activated DC populations.
Human DC Panel Kit
Human DC Panel Kit
Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This four‑color panel is designed for identifying conventional dendritic cells (cDCs) and evaluating their maturation and activation status in human peripheral blood or other biological samples. HLA‑DR and CD11c were included to identify conventional dendritic cells. CD80 and CD86 are markers often used to assess DC maturation and activation; positivity for either marker indicates an activated state, and the combined expression pattern can further distinguish mature DC subsets. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest, such as CD83 or CD40, for more detailed functional characterization.
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