Human Cytokine 10-Plex Assay (适用于Luminex)

Catalog Number: S0X1001 Reactivity: Human Conjugation: Brand: Starter

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$4,000 USD

Size: 1x96T

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Product Details

Product Specification

Antigen	IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IL-13、TNF-α、IFN-γ
Antibody Type	Recombinant mAb
Reactivity	Human
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8℃ as supplied.

Kit

Precision	批内变异系数 (Intra-assay): 2.67%-7.76%；批间变异系数 (Inter-assay): 8.3%-13.76%
Sample type	Cell culture supernatant, Serum, Plasma
Assay type	Sandwich (Qualitative)
Sensitivity	<1.0pg/ml
Range	0.5-10000 pg/mL
Recovery	70%-130%
Assay time	70 minutes
Species reactivity	Human
Plate	Non-detachable plate

Background

xMAP Technology uses colored beads to perform biological assays similar to ELISA or nucleic acid hybridization assays. By color-coding microscopic beads into many spectrally distinct sets, each bead set can be coated with a nucleic acid or protein capture molecule specific to a particular biological target, allowing the simultaneous capture of multiple analytes from a single sample. Because of the microscopic size and low density of these beads, assay reactions exhibit virtually solution-phase kinetics. However, once an assay is complete the solid phase characteristics allow each bead to be analyzed discretely. By incorporating magnetic properties into xMAP microspheres, assay washing is simplified while maintaining desirable solution-phase properties.

Picture

Luminex

Linearity
After serial dilution of the high-concentration combined proteins of IL-1β，IL-2, IL-4, IL-5, IL-6, IL-8,IL-10, IL-13,TNF-α,and IFN-γusing plasma, serum, and cell supernatant matrices respectively, the linear slopes between the actual detected values and the theoretical values are as shown in the table.

Standard Curve
Example of IL-1β, IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-13,TNF-α,IFN-γstandard curve in Assay Diluent.

Performance Characteristics

Precision
Ten replicates of each of three different levels of IL-1β, IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-13,TNF-α,IFN-γwere tested.

Specificity
The antibodies used in the kit have been screened for their specific reactivity with particular cytokines. After analyzing samples containing only a single recombinant cytokine protein, it was found that when using this detection method, there was no cross-reaction or background detection of cytokines in other capture magnetic bead groups.

Recovery
Within the detection range, the combined cytokine proteins were added to serum,plasma,cell supernatant at high concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. Theresults were compared with those of samples that had the same concentration of cytokines added in the assay buffer.