Human CXCL1/GRO α culture media OneStep ELISA Kit

Catalog Number: S0C3240 Reactivity: Hu Conjugation: Brand: Starter

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$200 USD

Size: 1x48T 1x96T 1x24T 5x96T 10x96T

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Product Details

Product Specification

Antigen	CXCL1
Immunogen	Recombinant Protein
Antibody Type	Recombinant mAb
Reactivity	Hu
Purification	Protein A
Stability & Storage	12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

Kit

Precision	Intra-assay: 3.0% Inter-assay: 3.6%
Sample type	Cell culture supernatant
Assay type	Sandwich (quantitative)
Sensitivity	0.55 pg/mL
Range	4.7 pg/mL – 300 pg/mL
Recovery	Cell culture supernatant: 98%
Assay time	60 minutes

Background

Human CXCL1, also known as Growth-regulated alpha protein (GRO-α) or Melanoma Growth Stimulatory Activity (MGSA), is a small cytokine belonging to the CXC chemokine family. It is a secreted growth factor that signals primarily through the G-protein coupled receptor CXCR2, and at higher concentrations can also activate CXCR1. The protein's main function is to act as a potent chemoattractant for neutrophils, playing a critical role in inflammation and innate immune responses. Notably, naturally occurring processed forms of CXCL1 (such as GRO-alpha 4-73) exhibit a 30-fold higher chemotactic activity compared to the full-length protein. Recombinant human CXCL1 is commercially available in various forms, including a 73-amino-acid variant with a predicted molecular mass of 7.9 kDa, as well as tagged versions for research applications. Beyond its role in inflammation, aberrant expression of CXCL1 is strongly associated with the growth and progression of certain tumors, making it a target of interest in cancer research.

Picture

ELISA

Standard curve
Example of Human CXCL1 standard curve in Assay Diluent AE3.

Spike Recovery
The recovery of Human CXCL1 was evaluated in activated samples spiked with concentrations spanning the entire assay range.

Spike-and-dilution Linearity
To evaluate assay linearity, culture media sample was spiked with high levels of Human CXCL1 in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.

Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGE

HOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.