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Human CFB/Complement factor B Protein, His Tag

Human CFB/Complement factor B Protein, His Tag

Catalog Number: S0A0218 Brand: Starter
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Product Details

Product Specification


Species Human
Synonyms C3/C5 convertase, Glycine-rich beta glycoprotein (GBG), PBF2, Properdin factor B, BF, BFD
Accession P00751-1
Amino Acid Sequence

Protein sequence (P00751-1, Thr26-Leu764, with C-His Tag) TPWSLARPQGSCSLEGVEIKGGSFRLLQEGQALEYVCPSGFYPYPVQTRTCRSTGSWSTLKTQDQKTVRKAECRAIHCPRPHDFENGEYWPRSPYYNVSDEISFHCYDGYTLRGSANRTCQVNGRWSGQTAICDNGAGYCSNPGIPIGTRKVGSQYRLEDSVTYHCSRGLTLRGSQRRTCQEGGSWSGTEPSCQDSFMYDTPQEVAEAFLSSLTETIEGVDAEDGHGPGEQQKRKIVLDPSGSMNIYLVLDGSDSIGASNFTGAKKCLVNLIEKVASYGVKPRYGLVTYATYPKIWVKVSEADSSNADWVTKQLNEINYEDHKLKSGTNTKKALQAVYSMMSWPDDVPPEGWNRTRHVIILMTDGLHNMGGDPITVIDEIRDLLYIGKDRKNPREDYLDVYVFGVGPLVNQVNINALASKKDNEQHVFKVKDMENLEDVFYQMIDESQSLSLCGMVWEHRKGTDYHKQPWQAKISVIRPSKGHESCMGAVVSEYFVLTAAHCFTVDDKEHSIKVSVGGEKRDLEIEVVLFHPNYNINGKKEAGIPEFYDYDVALIKLKNKLKYGQTIRPICLPCTEGTTRALRLPPTTTCQQQKEELLPAQDIKALFVSEEEKKLTRKEVYIKNGDKKGSCERDAQYAPGYDKVKDISEVVTPRFLCTGGVSPYADPNTCRGDSGGPLIVHKRSRFIQVGVISWGVVDVCKNQKRQKQVPAHARDFHINLFQVLPWLKEKLQDEDLGFL
Expression System HEK293
Molecular Weight Predicted MW: 84.7 kDa Observed MW: 96 kDa
Purity ＞95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Unconjugated
Physical Appearance Lyophilized powder
Storage Buffer

Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4 with 3% trehalose.
Reconstitution Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation.
Stability & Storage

12 months from date of receipt, -20 to -70 °C as supplied.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
Please avoid repeated freeze-thaw cycles.

Background

Complement Factor B is a pivotal zymogen in the alternative pathway of the complement system. This serine protease circulates in plasma and is essential for innate immunity. Its activation occurs when it binds to C3b, forming the C3 convertase (C3bBb) upon cleavage by Factor D. This enzyme complex amplifies the complement response by cleaving more C3, driving opsonization, inflammation, and the formation of the membrane attack complex. Factor B also plays a role in the lectin pathway. As a key regulator, its deficiency or dysfunction is linked to susceptibility to infections and autoimmune diseases like atypical hemolytic uremic syndrome.

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SDS-PAGE

2μg（R: reducing conditions）