Standard curve
Example of Human CCL22/MDC standard curve in Assay Diluent D1.
Human CCL22/MDC OneStep ELISA Kit
Human CCL22/MDC OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | CCL22 |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8°C as supplied. |
Kit
| Precision | Intra-assay: 2.4% Inter-assay: 3.5% |
| Sample type | Cell culture supernatant; Serum; Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.162 pg/mL |
| Range | 6.25 pg/mL – 400 pg/mL |
| Recovery | Cell culture supernatant: 104% Serum: 104% EDTA Plasma: 107% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Human CCL22 (C-C motif chemokine ligand 22), also known as macrophage-derived chemokine (MDC), is a small secretory protein composed of approximately 67-69 amino acids. Its gene is located on chromosome 16. It exerts its biological functions primarily by interacting with its receptor CCR4, thereby chemotactically recruiting various immune cells, including regulatory T cells (Tregs), Th2 cells, and natural killer (NK) cells, playing a crucial role in maintaining immune homeostasis and mediating inflammatory responses.
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ELISA
Linearity
The concentrations of Human CCL22 were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 189.1 pg/mL in stimulated human PBMC supernatant.
#2 sample is undiluted samples are as follows: human PBMC cells culture for 5days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 162.96 pg/mL in unstimulated human PBMC supernatant.
#3 sample is undiluted samples are as follows: Human serum was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1041.61 pg/mL in human serum.
#4 sample is undiluted samples are as follows: Human plasma-EDTA was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 399.44 pg/mL in human plasma-EDTA.
Protocol Diagram
