Immobilized Recombinant Human S100A9 at 10 μg/mL (50 μL/well) can bind Human Calprotectin (S100A8), His tag with EC50 of 18.53-25.26 ng/ml.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen (CFAG); Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8 (MRP-8; p8); Urinary stone protein band A; CAGA |
Accession | P05109 |
Amino Acid Sequence | Protein sequence(P05109, Met1-Glu93, with C-10*His) |
Expression System | E.coli |
Molecular Weight | Predicted MW: 12.5 kDa Observed MW: 13.0 kDa |
Purity | >95% by SDS-PAGE |
Endotoxin | <1EU/μg |
Tag | His Tag |
Physical Appearance | Lyophilized Powder |
Storage Buffer | Lyophilized from a 0.2 μm filtered solution of 0.2M PBS, pH7.4. |
Reconstitution | Reconstitute no more than 1 mg/mL according to the size in deionized water after rapid centrifugation. |
Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied. 6 months, -20 to -70 °C under sterile conditions after reconstitution. 1 week, 2 to 8 °C under sterile conditions after reconstitution. Please avoid repeated freeze-thaw cycles. |
Background
S100A8 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis and post COVID-19 condition.
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Bioactivity
SDS-PAGE

2μg(R: reducing conditions)

