Human PBMCs were stained using the Human B Cell panel consisting of anti-human CD19 FITC, anti-human CD20 Pacific Blue, anti-human CD138 PE, anti-human CD24 Alexa Fluor® 647 and anti-human CD38 PE-Cy7 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD19 FITC-A vs CD20 Pacific Blue plot show the Mature B cells with the Fixable Viability Dye 545- gate applied.
4. Create a CD19 FITC-A vs CD24 Alexa Fluor® 647 dot show the immature B cells, epithelial cells and tissue-resident precursor cells with the Fixable Viability Dye 545- gate applied.
5. Create a CD138 PE-A vs CD38 PE-Cy7-A dot plot and apply the CD19- gate. Create gates to encompass CD138+CD38+ cells (Plasma cells), CD38+CD138- cells (Activated B cells /plasmablasts), and CD38-CD138- cells (Resting mature B cells)
6. Create a CD38 PE-Cy7-A vs CD24 Alexa Fluor® 647-A dot plot and apply the CD19+ gate. Create gates to encompass CD38+CD24+ cells (transitional B cells), CD38+CD24- cells (plasmablasts), and CD38-CD138- cells (Resting mature B cells)
Human B Cell Panel Kit
Human B Cell Panel Kit
Product Details
Product Details
Product Specification
| Application | FCM |
| Reactivity | Hu |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.09% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Background
This five‑color panel is designed for characterizing B‑cell subsets and plasma cells in human peripheral blood or other biological samples, with a focus on differentiation stages from immature to mature B lymphocytes and terminally differentiated antibody‑secreting cells. CD19 was included as a pan‑B‑cell marker to identify the total B‑cell population, and CD20 was included to distinguish mature B cells from plasma cells, as CD20 is downregulated upon terminal differentiation. CD138 is a marker often used to detect plasma cells; when combined with CD38, which is highly expressed on activated B cells and plasmablasts, the panel allows the discrimination of plasma cells, activated B cells/plasmablasts, and resting mature B cells. Furthermore, the expression pattern of CD38 and CD24 on B cells can identify transitional B cells, plasmablasts, and resting mature B cells. The panel is compatible with most standard three‑laser flow cytometers. If desired, the panel can serve as a backbone B‑cell tube, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest for more refined subsetting.
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