Standard curve
Example of Human Apolipoprotein E/APOE standard curve in Assay Diluent EJ.
Human Apolipoprotein E/APOE Serum & Plasma Onestep ELISA Kit
Human Apolipoprotein E/APOE Serum & Plasma Onestep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | Apolipoprotein E/APOE |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.8%； Inter-assay: 4.1% |
| Sample type | Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.482 ng/mL |
| Range | 0.625 ng/mL – 40 ng/mL |
| Recovery | Serum: 101% Plasma: 104% |
| Assay time | 60 minutes |
| Species reactivity | Hu |
Background
Human APOE (Apolipoprotein E) is a key protein involved in lipid metabolism, primarily produced in the liver and brain. It functions as a carrier for cholesterol and other fats, facilitating their transport through the bloodstream. APOE exists in three common isoforms—APOE2, APOE3, and APOE4—which differ by single amino acids. Among these, APOE4 is the strongest genetic risk factor for late-onset Alzheimer's disease, while APOE2 appears to offer some protective effects. The protein also plays a crucial role in neuronal repair and inflammation regulation within the central nervous system. Its interaction with cellular receptors allows cells to take up lipids for membrane maintenance and energy. Overall, APOE is essential for both cardiovascular and brain health, with its genetic variations significantly influencing disease susceptibility.
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ELISA
Linearity
The concentrations of Human Apolipoprotein E/APOE were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human plasma was 1000fold dilution (0.1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 21.40 μg/mL in stimulated human plasma.
Sample is undiluted samples are as follows: human serum was 1000fold dilution (0.1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 22.97 μg/mL in stimulated human serum.
To assess the linearity of the assay, sample was spiked with high concentrations of Human Apolipoprotein E/APOE in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Protocol Diagram
