Human Angiotensinogen/AGT/SerpinA8 ELISA Kit(Plus)

Detection principle:

This kit adopts double antibody sandwich enzyme-linked immunosorbent detection technology. Specific anti-human AGT antibodies were pre-coated on high affinity plates. The standard substance, the detection sample and the detection antibody labeled with horseradish peroxidase are added to the wells of the enzyme labeled plate, and after incubation, the AGT present in the sample binds to the solid phase antibody and the detection antibody. After washing, TMB, a chromogenic substrate, was added to protect the color from light. The depth of the color reaction is proportional to the concentration of AGT in the sample. The reaction was stopped by adding a stop solution and the absorbance value was measured at a wavelength of 450 nm (reference wavelength of 570-630 nm).

Test Type:Double antibody sandwich method

Form:Pre-coated 96-well plate

Test Sample Type:Cell supernatant, serum, plasma

Sample Load:100 μl

Kit Components:One copy of the pre-coated 96-well plate, standard, Human AGT test antibody, test buffer, TMB chromogenic substrate, wash solution, stop solution, plate sealing membrane, and instructions.

Sensitivity:19.93 pg/mL

Detection range:0.31-20ng/ml

Recovery Range:83-105%

Save Method:2-8℃

Standard graph:

Background:

Angiotensinogen (AGT), also known as SerpinA8, is a member of the family of serine protease inhibitors. AGT is an alpha-2-globulin that is constitutively produced by the liver and released into the blood circulation. AGT is an essential component of the renin-angiotensin system and is an effective blood pressure regulator. AGT can be cleaved into 3 chains: angiotensin-1, angiotensin-2, and angiotensin-3.

Loss of AGT is associated with essential hypertension and renal tubular hypoplasia. Several serine protease inhibitors (antithrombin, maspin, pigment epithelium-derived factors, and kallistatin) have recently been shown to have anti-angiogenic activity, suggesting a common mechanism of endothelial cell proliferation and migration. AGT and its renin cleavage products, in the range of plasma concentrations observable in vitro and in vivo, are also inhibitors of angiogenesis.