WB result of HTR2A Recombinant Rabbit mAb
Primary antibody: HTR2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
HTR2A Recombinant Rabbit mAb (S-4426-14)
HTR2A Recombinant Rabbit mAb (S-4426-14)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | HTR2A |
| Synonyms | 5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2AAlternative nameSerotonin receptor 2A; HTR2 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Synapse |
| Accession | P28223 |
| Clone Number | S-4426-14 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | Raji, U-87, mouse heart, mouse cerebellum, mouse brain, rat heart, rat cerebellum, rat brain, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt, Mk |
| ICC | 1:500 | Hu |
Background
HTR2A, also known as the 5-hydroxytryptamine receptor 2A, is a G protein-coupled receptor composed of 471 amino acids with a molecular weight of approximately 52 kDa. It serves as a key receptor for the neurotransmitter serotonin (5-hydroxytryptamine) in the brain and is widely distributed in the cerebral cortex and basal ganglia, as well as in smooth muscle cells of peripheral tissues. When activated by serotonin or psychoactive substances such as LSD and psilocybin, HTR2A primarily couples to the Gq protein, activating phospholipase C and triggering a cascade of intracellular calcium release and downstream signaling pathways, thereby modulating neural activity, perception, cognition, and emotion. Consequently, dysfunction of HTR2A is closely associated with various psychiatric disorders, including schizophrenia, depression, and substance dependence, making it an important therapeutic target for the development of antipsychotic and antidepressant drugs.
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Western Blot
WB result of HTR2A Recombinant Rabbit mAb
Primary antibody: HTR2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Lane 2: mouse cerebellum lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
WB result of HTR2A Recombinant Rabbit mAb
Primary antibody: HTR2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Lane 2: rat cerebellum lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
WB result of HTR2A Recombinant Rabbit mAb
Primary antibody: HTR2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
Immunocytochemistry
ICC shows positive staining in U-87 MG cells. Anti-HTR2A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
