WB result of HSP90 Recombinant Rabbit mAb
Primary antibody: HSP90 Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: LnCap whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
HSP90 Recombinant Rabbit mAb (S-5412)
HSP90 Recombinant Rabbit mAb (S-5412)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | HSP90 |
| Synonyms | Heat shock protein HSP 90-alpha; Heat shock 86 kDa (HSP 86; HSP86); Heat shock protein family C member 1; Lipopolysaccharide-associated protein 2 (LAP-2; LPS-associated protein 2); Renal carcinoma antigen NY-REN-38; HSP90A; HSPC1; HSPCA; HSP90AA1 |
| Location | Cytoplasm, Nucleus, Cell membrane |
| Accession | P07900 |
| Clone Number | S-5412 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HEK-293, MCF7, Jurkat, LnCaP, HepG2, NIH/3T3, RAW264.7, mouse brain, C6, PC-12, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
| IHC-P | 1: 2000 | Hu, Ms, Rt |
Background
HSP90 (Heat Shock Protein 90) is one of the most abundant and functionally critical molecular chaperones in eukaryotic cells, belonging to the HSP90 family (which in mammals mainly comprises cytosolic HSP90α and HSP90β, endoplasmic reticulum-resident GRP94, and mitochondrial TRAP1). Its core function is to act as a "conformational switch" that, through ATP-dependent conformational changes, assists its "client proteins"—numbering in the hundreds, the vast majority of which are involved in signal transduction (such as kinases, steroid hormone receptors, and transcription factors)—in proper folding, maturation, and maintenance of active conformations, while also preventing these client proteins from being degraded due to misfolding. HSP90 does not work in isolation; it collaborates with multiple co-chaperones (such as HSP70, HSP40) and immunophilins to form dynamic multi-subunit complexes, orchestrating a precise "folding cycle" in which it alternates between open and closed conformations driven by ATP hydrolysis, thereby capturing, loading, and releasing substrate proteins. This process is particularly crucial under cellular stress conditions (such as heat shock, oxidative stress, or heavy metal exposure), when HSP90 expression is upregulated to protect the proteome from damage, while it also participates in important physiological and pathological processes including cell cycle regulation, cell survival, and tumorigenesis. Precisely because of its central role in maintaining the stability of multiple oncogenic proteins, HSP90 has become an important target in cancer therapy; various natural-product-derived inhibitors (such as geldanamycin derivatives, radicicol, and resorcinol compounds) have been developed and entered clinical studies, aiming to simultaneously degrade multiple oncogenic client proteins by blocking its ATPase activity, demonstrating broad-spectrum antitumor potential—however, their clinical application remains challenged by side effects including hepatotoxicity, ocular toxicity, and heat shock response rebound.
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Western Blot
WB result of HSP90 Recombinant Rabbit mAb
Primary antibody: HSP90 Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Raw 264.7 whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
WB result of HSP90 Recombinant Rabbit mAb
Primary antibody: HSP90 Recombinant Rabbit mAb at 1/20000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: PC-12 whole cell lysate 20 µg
Lane 3: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-HSP90 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-HSP90 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-HSP90 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-HSP90 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-HSP90 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-HSP90 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-HSP90 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
