WB result of HSP90 alpha/beta Recombinant Mouse mAb
Primary antibody: HSP90 alpha/beta Recombinant Mouse mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: K562 whole cell lysate 20 µg
Lane 5: IMR-32 whole cell lysate 20 µg
Lane 6: SK-BR-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 90 kDa
HSP90 alpha/beta Recombinant Mouse mAb (S-5282)
HSP90 alpha/beta Recombinant Mouse mAb (S-5282)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | HSP90 alpha/beta |
| Synonyms | Heat shock protein HSP 90-alpha; Heat shock 86 kDa (HSP 86; HSP86); Heat shock protein family C member 1; Lipopolysaccharide-associated protein 2 (LAP-2; LPS-associated protein 2); Renal carcinoma antigen NY-REN-38; HSP90A; HSPC1; HSPCA; HSP90AA1; Heat shock protein HSP 90-beta; Heat shock 84 kDa (HSP 84; HSP84); Heat shock protein family C member 3; HSP90AB1; HSP90B; HSPC2; HSPC3; HSPCB |
| Location | Cytoplasm, Nucleus |
| Accession | P07900、P08238 |
| Clone Number | S-5282 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a,k |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, A431, HepG2, K562, IMR-32, SK-BR-3, 3T3-L1, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
HSP90α and HSP90β are highly conserved molecular chaperones that facilitate the folding, stabilization, and activation of diverse client proteins—including kinases, transcription factors, and hormone receptors—thereby maintaining cellular proteostasis and signal transduction. While sharing ~85% sequence identity and a common domain architecture, the inducible HSP90α and constitutively expressed HSP90β exhibit distinct tissue distributions and functional specializations in stress responses and oncogenic pathways.
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Western Blot
WB result of HSP90 alpha/beta Recombinant Mouse mAb
Primary antibody: HSP90 alpha/beta Recombinant Mouse mAb at 1/1000 dilution
Lane 1: 3T3-L1 whole cell lysate 20 µg
Lane 2: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 90 kDa
WB result of HSP90 alpha/beta Recombinant Mouse mAb
Primary antibody: HSP90 alpha/beta Recombinant Mouse mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 84 kDa
Observed MW: 90 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver cancer. Anti-HSP90 alpha/beta antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-HSP90 alpha/beta antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-HSP90 alpha/beta antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-HSP90 alpha/beta antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-HSP90 alpha/beta antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-HSP90 alpha/beta antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-HSP90 alpha/beta antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-HSP90 alpha/beta antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
