WB result of HSP60 Recombinant Mouse mAb
Primary antibody: HSP60 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: SW480 whole cell lysate 20 µg
Lane 6: K562 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 60 kDa
HSP60 Recombinant Mouse mAb (S-5385)
HSP60 Recombinant Mouse mAb (S-5385)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | HSP60 |
| Synonyms | Heat shock 70 kDa protein 14; HSP70-like protein 1; Heat shock protein HSP60; Heat shock protein family A member 14; HSP70L1; HSPA14 |
| Location | Cytoplasm |
| Accession | Q0VDF9 |
| Clone Number | S-5385 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, HEK-293, Jurkat, MCF7, SW480, K562, NIH/3T3, Neuro-2a, mouse brain, mouse heart, PC-12, rat brain, rat heart, COS-7 |
| Purification | Protein G |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
HSP60 is a highly conserved and critically important molecular chaperone in cells. Its core function is to assist in the proper folding and assembly of nascent polypeptide chains, as well as the refolding of denatured proteins under stress conditions, thereby maintaining intracellular protein homeostasis. HSP60 typically exists as a double-ring oligomeric structure composed of 14 subunits (i.e., a "barrel-shaped" structure), and through ATP hydrolysis-driven conformational changes, it binds to the hydrophobic surfaces of substrate proteins to provide an isolated microenvironment that promotes the formation of correct conformations. In addition to its classical chaperone functions in the cytoplasm and mitochondria, HSP60 is also involved in the transmembrane transport of mitochondrial proteins, and accumulating evidence indicates that it can be secreted extracellularly or appear on the cell surface, exerting non-classical functions such as immunomodulation and induction of inflammatory signaling. HSP60 expression is significantly upregulated under stress conditions such as heat shock and oxidative stress. Its dysfunction is not only associated with various neurodegenerative diseases and heart diseases, but its expression levels are also markedly altered in multiple tumors, including breast cancer and colorectal cancer, closely correlating with malignant progression and drug resistance.
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Western Blot
WB result of HSP60 Recombinant Mouse mAb
Primary antibody: HSP60 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: Neuro-2a whole cell lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Lane 4: mouse heart lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 60 kDa
WB result of HSP60 Recombinant Mouse mAb
Primary antibody: HSP60 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Lane 3: rat heart lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 60 kDa
WB result of HSP60 Recombinant Mouse mAb
Primary antibody: HSP60 Recombinant Mouse mAb at 1/10000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 55 kDa
Observed MW: 60 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-HSP60 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-HSP60 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-HSP60 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-HSP60 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-HSP60 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-HSP60 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-HSP60 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
