WB result of HSD3B1 Recombinant Rabbit mAb
Primary antibody: HSD3B1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse testis lysate 25 µg
Lane 2: mouse placenta lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 40 kDa
HSD3B1 Recombinant Rabbit mAb
HSD3B1 Recombinant Rabbit mAb
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | HSD3B1 |
| Synonyms | 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I (3-beta-HSD I); 3-beta-hydroxy-5-ene steroid dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-hydroxysteroid 3-dehydrogenase; Delta-5-3-ketosteroid isomerase; Hsd3b; Hsd3b1 |
| Location | Mitochondrion, Endoplasmic reticulum |
| Accession | P24815 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms, Rt |
| Positive Sample | Mouse testis, mouse placenta, rat testis |
| Purification | Protein A |
| Concentration | 1.2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:500 | Ms, Rt |
Background
HSD3B1, a bifunctional enzyme with both dehydrogenase and isomerase activities that catalyzes the oxidation and isomerization of Δ5-3β-hydroxysteroid precursors into Δ4-ketosteroids, a key step in the biosynthesis of all classes of hormonal steroids. The enzyme is a single-pass transmembrane protein of approximately 42 kDa localized to the endoplasmic reticulum and mitochondrial membranes. In humans, the type 1 isoenzyme encoded by HSD3B1 is expressed mainly in the placenta and peripheral tissues, whereas steroidogenesis in the adrenal glands, ovaries, and testes relies primarily on the type 2 isoenzyme encoded by HSD3B2. In recent years, HSD3B1 has attracted widespread attention in the field of prostate cancer: it is the rate-limiting enzyme for extragonadal androgen synthesis, converting adrenal-derived dehydroepiandrosterone (DHEA) to androstenedione and subsequently to testosterone and dihydrotestosterone. Its common germline variant 1245A>C (rs1047303) causes the N367T amino acid change, rendering the enzyme protein resistant to ubiquitin-mediated degradation and increasing its intracellular steady-state level, thereby enhancing intratumoral androgen synthesis, promoting the development of castration-resistant prostate cancer, and shortening the duration of response to androgen deprivation therapy. In addition, a 2023 study found that placental HSD3B1 possesses oxysterol 3β-hydroxysteroid dehydrogenase activity and may be involved in regulating the levels of bioactive oxysterols transferred to the fetus.
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Western Blot
WB result of HSD3B1 Recombinant Rabbit mAb
Primary antibody: HSD3B1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat testis lysate 25 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 42 kDa
Observed MW: 42 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse testis. Anti-HSD3B1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse placenta. Anti-HSD3B1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat ovary. Anti-HSD3B1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat placenta. Anti-HSD3B1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
