WB result of HPRT1 Rabbit pAb
Primary antibody: HPRT1 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
HPRT1 Rabbit Polyclonal Antibody
HPRT1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | HPRT1 |
| Synonyms | Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase; HPRT |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P00492 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, A549, A431, MCF7, HepG2, mouse brain, rat brain |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu |
Background
HPRT1 (hypoxanthine phosphoribosyltransferase 1) is an enzyme that plays a crucial role in the purine salvage pathway, which is essential for the recycling of purine nucleotides. It catalyzes the conversion of hypoxanthine to inosine monophosphate (IMP) and guanine to guanosine monophosphate (GMP) by transferring the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This process is vital for maintaining the supply of purine nucleotides needed for DNA and RNA synthesis, as well as for energy production in cells. Mutations in the HPRT1 gene can lead to conditions such as Lesch-Nyhan syndrome, characterized by the accumulation of uric acid due to impaired purine metabolism. Additionally, HPRT1 is implicated in various cancers, where its overexpression can promote cell proliferation and contribute to drug resistance.
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Picture
Western Blot
WB result of HPRT1 Rabbit pAb
Primary antibody: HPRT1 Rabbit pAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
WB result of HPRT1 Rabbit pAb
Primary antibody: HPRT1 Rabbit pAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-HPRT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-HPRT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-HPRT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-HPRT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-HPRT1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
