HPD Recombinant Rabbit mAb (S-4536-65)

Catalog Number: S0B60401 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	HPD
Synonyms	4-hydroxyphenylpyruvate dioxygenase; 4-hydroxyphenylpyruvic acid oxidase (4HPPD; HPD; HPPDase); N(6)-adenosine-methyltransferase HPD; PPD
Immunogen	Synthetic Peptide
Location	Cytoplasm, Nucleus
Accession	P32754
Clone Number	S-4536-65
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	HeLa, HepG2, A-204
Predicted Reactivity	Bv, Pg
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu
IHC-P	1:1000	Hu

Background

HPD is a key enzyme in the human body responsible for catalyzing the second step of the tyrosine catabolic pathway, converting 4-hydroxyphenylpyruvate into homogentisate. This iron-containing homodimeric protein is most abundantly expressed in human liver and kidney, and is also present in neurons of the cerebral cortex. The physiological function of HPD is crucial; defects in its encoding gene lead to autosomal recessive tyrosinemia type III, characterized by elevated blood tyrosine levels in patients, accompanied by mild intellectual disability and seizures. In addition, specific mutations in this gene can cause another rare tyrosine metabolic disorder—Hawkinsinuria. Beyond its classical metabolic function, recent studies have revealed that HPD is also a copper-binding protein. In colorectal cancer, it responds to copper stress by translocating to mitochondria and interacting with the DLAT protein, delivering copper ions to DLAT to trigger a form of cell death known as "cuproptosis," thereby playing a key role in tumor suppression.

Picture

Western Blot

WB result of HPD Recombinant Rabbit mAb
Primary antibody: HPD Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A-204 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 39 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-HPD antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-HPD antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver cancer. Anti-HPD antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.