Hormone sensitive lipase/HSL Recombinant Rabbit mAb (S-6116)

Catalog Number: S0B60740 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Hormone sensitive lipase/HSL
Synonyms	Hormone-sensitive lipase; Monoacylglycerol lipase LIPE; Retinyl ester hydrolase (REH); LIPE
Location	Cytoplasm, Cell membrane
Accession	Q05469
Clone Number	S-6116
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	Raji, mouse adipose
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms
IHC-P	1:2000	Hu, Ms, Rt

Background

Hormone-sensitive lipase (HSL) is an intracellular neutral lipase that was long regarded as the rate-limiting enzyme for triglyceride hydrolysis in adipose tissue; it is now known to act primarily at the diacylglycerol step of the lipolytic cascade, hydrolyzing diacylglycerol to monoacylglycerol. HSL activity is acutely regulated by reversible phosphorylation: catecholamines and other hormones phosphorylate HSL via the cAMP/PKA pathway, promoting its translocation to lipid droplets where it exerts its hydrolytic action, whereas insulin inhibits this process. The enzyme has an unusually broad substrate specificity, hydrolyzing not only triglycerides, diacylglycerols, and monoacylglycerols but also cholesterol esters, steroid fatty acid esters, and retinyl esters. HSL is highly expressed in adipose tissue and steroidogenic tissues, and is also expressed in cardiac muscle, skeletal muscle, macrophages, and pancreatic islets; in steroidogenic tissues it is mainly responsible for converting cholesterol esters into free cholesterol for hormone synthesis. At the pathophysiological level, HSL expression and activity correlate significantly with the maximal lipolytic capacity of human adipocytes, and abnormalities in its activity are involved in obesity, insulin resistance, and the excessive release of free fatty acids in diabetic ketoacidosis.

Picture

Western Blot

WB result of Hormone sensitive lipase/HSL Recombinant Rabbit mAb
Primary antibody: Hormone sensitive lipase/HSL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Huh7 whole cell lysate 20 μg
Lane 2: Raji whole cell lysate 20 μg
Negative control: Huh7 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 117 kDa
Observed MW: 100 kDa

WB result of Hormone sensitive lipase/HSL Recombinant Rabbit mAb
Primary antibody: Hormone sensitive lipase/HSL Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 μg
Lane 2: mouse adipose lysate 20 μg
Negative control: mouse liver lysate
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 117 kDa
Observed MW: 84, 36 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human adipose tissue. Anti-Hormone sensitive lipase/HSL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti- Hormone sensitive lipase/HSL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse adipose tissue. Anti-Hormone sensitive lipase/HSL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-Hormone sensitive lipase/HSL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat adipose tissue. Anti-Hormone sensitive lipase/HSL antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.