WB result of Homer1 Rabbit pAb
Primary antibody: Homer1 Rabbit pAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse brain lysate 20 µg
Negative control: mouse lung lysate; mouse liver lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 45 kDa
Homer1 Rabbit Polyclonal Antibody
Homer1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Homer1 |
| Synonyms | Homer protein homolog 1; Homer-1; SYN47 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q86YM7 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms, Rt |
| Positive Sample | mouse brain, rat brain |
| Predicted Reactivity | Bv |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
Background
Homer1 protein is a scaffolding molecule that plays a crucial role in modulating synaptic plasticity and signal transduction within neurons. It interacts with group 1 metabotropic glutamate receptors (mGluRs) and other synaptic proteins, influencing their localization, signaling, and trafficking. Homer1 consists of different isoforms, including Homer1a, which acts as an immediate early gene product and can regulate synaptic function and plasticity by altering the interaction between mGluRs and other proteins. This protein is also implicated in various neurological conditions due to its role in synaptic processes and its ability to affect neuronal excitability and neurotransmission.
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Western Blot
WB result of Homer1 Rabbit pAb
Primary antibody: Homer1 Rabbit pAb at 1/1000 dilution
Lane 1: rat lung lysate 20 µg
Lane 2: rat liver lysate 20 µg
Lane 3: rat brain lysate 20 µg
Negative control: rat lung lysate; rat liver lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 45 kDa
