HO-1 Rabbit Polyclonal Antibody

Catalog Number: S0B6935 Application: WB, IHC-P Reactivity: Hu, Ms Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	HO-1
Synonyms	Heme oxygenase 1; HO-1Cleaved into 1 chainsHeme oxygenase 1 soluble form; HO; HO1; HMOX1
Immunogen	Recombinant Protein
Location	Endoplasmic reticulum
Accession	P09601
Antibody Type	Polyclonal antibody
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms
Positive Sample	HeLa (treated with 50 µM arsenite for 8 hours), NIH/3T3 (treated with 50 µM arsenite for 8 hours)
Purification	Immunogen Affinity
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	Hu, Ms
IHC-P	1:1000	Hu, Ms

Background

Heme Oxygenase-1 (HO-1, HSP32) is a 32 kDa stress-responsive enzyme anchored to the smooth endoplasmic reticulum that cleaves heme at the α-meso carbon to release equimolar biliverdin (rapidly reduced to bilirubin), carbon monoxide (CO) and ferrous iron, thereby not only eliminating the pro-oxidant heme but also generating potent antioxidants and the signaling gas CO; transcription of the HMOX1 gene is potently induced by myriad stressors—including heme itself, ROS, heavy metals, cytokines, hypoxia, nitric oxide, and natural electrophiles—via Nrf2/ARE, Bach1, HIF-1, AP-1 and NF-κB pathways, giving HO-1 a central cytoprotective role against oxidative and inflammatory injury in cardiovascular, neuronal, renal and hepatic tissues, modulating mitochondrial biogenesis, autophagy and immune tolerance, while its products bilirubin and CO respectively scavenge radicals and activate cGMP/PKG signaling to vasodilate, inhibit platelet aggregation and suppress apoptosis, making HO-1 a therapeutic target in sepsis, ischemia–reperfusion, transplant rejection, metabolic disorders and neurodegeneration.

Picture

Western Blot

WB result of HO-1 Rabbit pAb
Primary antibody: HO-1 Rabbit pAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 50 µM arsenite for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa

WB result of HO-1 Rabbit pAb
Primary antibody: HO-1 Rabbit pAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 50 µM arsenite for 8 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 30 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse lung. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-HO-1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.