WB result of HNRNPL Rabbit pAb
Primary antibody: HNRNPL Rabbit pAb at 1/1000 dilution
Lane 1: Ramos whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Lane 3: HT-1080 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: PC-3 whole cell lysate 20 µg
Lane 6: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
HNRNPL Rabbit Polyclonal Antibody
HNRNPL Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | HNRNPL |
| Synonyms | Heterogeneous nuclear ribonucleoprotein L; HNRPL |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P14866 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | Ramos, PANC-1, HT-1080, HepG2, PC-3, HeLa, RAW264.7, C6, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:250 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
HNRNPL, also known as heterogeneous nuclear ribonucleoprotein L, is a ubiquitously expressed RNA-binding protein in cells that primarily participates in pre-mRNA processing, packaging, and alternative splicing regulation. As a core component of the hnRNP complex, it is typically localized in the nucleoplasm, preferentially binds to CA-rich sequences on RNA, and can act as either an activator or inhibitor of exon inclusion, playing a critical role in gene expression regulation. HNRNPL contains multiple RNA recognition motifs (RRMs), which constitute the structural basis for its RNA-binding activity, and its functions are not confined to the nucleus; due to its ability to shuttle between the nucleus and cytoplasm, it may also participate in the regulation of mRNA stability in the cytoplasm. Increasing research has also revealed that HNRNPL, through interactions with molecules such as long non-coding RNAs (lncRNAs), plays important roles in the development, progression, and chemoresistance of various cancers (including bladder cancer, esophageal cancer, ovarian cancer, and colorectal cancer), making it a potential disease biomarker and therapeutic target.
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Western Blot
WB result of HNRNPL Rabbit pAb
Primary antibody: HNRNPL Rabbit pAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
WB result of HNRNPL Rabbit pAb
Primary antibody: HNRNPL Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
WB result of HNRNPL Rabbit pAb
Primary antibody: HNRNPL Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-HNRNPL antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-HNRNPL antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-HNRNPL antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-HNRNPL antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-HNRNPL antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
