WB result of HNRNPA2B1 Mouse mAb
Primary antibody: HNRNPA2B1 Mouse mAb at 1/20000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K532 whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 36, 37 kDa
HNRNPA2B1 Mouse mAb
HNRNPA2B1 Mouse mAb
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | HNRNPA2B1 |
| Synonyms | Heterogeneous nuclear ribonucleoproteins A2/B1; HNRPA2B1 |
| Location | Cytoplasm, Nucleus, Secreted |
| Accession | P22626 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2a |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, HeLa, K532, HepG2, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 1.8 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:5000-1:50000 | Hu, Ms, Rt |
| IHC-P | 1:200-1:500 | Hu, Ms, Rt |
| ICC | 1:500-1:1000 | Hu, Ms, Rt |
Background
HNRNPA2B1 is a widely expressed RNA-binding protein in the human body, belonging to the hnRNP A/B subfamily. Its core function is to participate in the processing, transport, and metabolism of precursor mRNA, influencing gene expression regulation by binding to RNA to form ribonucleoprotein particles. This protein possesses a typical dual RNA recognition motif (RRM) domain, enabling it to specifically recognize and bind to specific sequences in RNA substrates, such as AGG and UAG motifs. HNRNPA2B1 is involved in mRNA splicing and nuclear export within the nucleus, and it has also been identified as a "reader" of N6-methyladenosine (m6A) modifications, recognizing primary miRNAs containing m6A marks and promoting their processing. Additionally, it participates in the cytoplasmic transport of specific mRNAs, the sorting of miRNAs into exosomes, and innate immune responses. Notably, aberrant expression or mutations of HNRNPA2B1 are associated with various diseases, including multiple cancers and inclusion body myopathy.
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Western Blot
WB result of HNRNPA2B1 Mouse mAb
Primary antibody: HNRNPA2B1 Mouse mAb at 1/20000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa
WB result of HNRNPA2B1 Mouse mAb
Primary antibody: HNRNPA2B1 Mouse mAb at 1/20000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 37 kDa
Observed MW: 37 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-HNRNPA2B1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in U-2 OS cells. Anti-HNRNPA2B1 antibody was used at 1/800 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-HNRNPA2B1 antibody was used at 1/800 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C6 cells. Anti-HNRNPA2B1 antibody was used at 1/800 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
