hnRNP A1 Recombinant Rabbit mAb

Catalog Number: S0B60697 Application: WB, IHC-P Reactivity: Hu, Ms, Rt Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	hnRNP A1
Location	Cytoplasm, Nucleus
Accession	P09651
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu, Ms, Rt
Positive Sample	HeLa, 293T, HepG2, U-2 OS, MCF7, RD, PC-3, Neuro-2a, C6
Purification	Protein A
Concentration	0.8 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:2000	Hu, Ms, Rt
IHC-P	1:100	Hu

Background

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is an abundant RNA-binding protein that serves as a core component of the hnRNP complex, participating in pre-mRNA processing. Its structure consists of two tandem N-terminal RNA recognition motifs (RRM1 and RRM2, collectively known as UP1) and a C-terminal glycine-rich domain, which contains RGG boxes and the M9 nuclear localization signal. hnRNP A1 recognizes YAG motifs in a sequence-specific manner, but its interaction mode with RNA is plastic, allowing it to bind both structured and unstructured RNA. Functionally, hnRNP A1 regulates splice site selection in alternative splicing by antagonizing SR proteins, and also participates in mRNA stability, nuclear export, and translation. Its C-terminal glycine-rich domain mediates protein-protein interactions and confers nucleic acid annealing activity on the protein. In addition, dysregulation of hnRNP A1 is associated with neurodegenerative diseases and viral infections, and disruption of its RNA-binding capacity has become a potential therapeutic target.

Picture

Western Blot

WB result of hnRNP A1 Recombinant Rabbit mAb
Primary antibody: hnRNP A1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 25 μg
Lane 2: 293T whole cell lysate 25 μg
Lane 3: Hep G2 whole cell lysate 25 μg
Lane 4: U-2 OS whole cell lysate 25 μg
Lane 5: MCF7 whole cell lysate 25 μg
Lane 6: RD whole cell lysate 25 μg
Lane 7: PC-3 whole cell lysate 20 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 34 kDa

WB result of hnRNP A1 Recombinant Rabbit mAb
Primary antibody: hnRNP A1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 34 kDa
This blot was developed with high sensitivity substrate

WB result of hnRNP A1 Recombinant Rabbit mAb
Primary antibody: hnRNP A1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 25 μg
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 38 kDa
Observed MW: 34 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human colon. Anti-hnRNP A1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-hnRNP A1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-hnRNP A1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human transitional cell carcinoma. Anti-hnRNP A1 antibody was used at 1/100 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.