HLA-ABC Recombinant Rabbit mAb (S-3995-2)

Catalog Number: S0B60106 Application: WB, IHC-P Reactivity: Hu Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100µl

Quantity:

For shipping services or bulk orders, you may request a quotation.

Secure checkout with

View full details

Product Details

Product Specification

Host	Rabbit
Antigen	HLA-ABC
Synonyms	HLA class I histocompatibility antigen, A alpha chain; Human leukocyte antigen A (HLA-A); HLAA; HLA-A; HLA class I histocompatibility antigen, B alpha chain; Human leukocyte antigen B (HLA-B); HLA-B; HLAB; HLA class I histocompatibility antigen, C alpha chain; HLA-C; HLA-Cw; Human leukocyte antigen C; HLA-C; HLAC
Immunogen	Recombinant Protein
Location	Cell membrane, Endoplasmic reticulum membrane
Accession	P04439、P01889、P10321
Clone Number	S-3995-2
Antibody Type	Recombinant mAb
Isotype	IgG
Application	WB, IHC-P
Reactivity	Hu
Positive Sample	Jurkat, HepG2, THP-1, Raji, Ramos
Purification	Protein A
Concentration	0.5 mg/ml
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.02% sodium azide
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500-1:1000	Hu
IHC-P	1:2000	Hu

Background

HLA-ABC (also termed HLA class I classical heavy chains A, B, and C) are 44 kDa type I transmembrane glycoproteins encoded within the major histocompatibility complex on chromosome 6p21.3; each non-covalently associates with the invariant light chain β2-microglobulin (12 kDa) to form a peptide-binding heterodimer that is constitutively expressed on nearly all nucleated cells. These molecules serve as the principal conduit for presenting endogenously derived 8–11-mer peptides to CD8⁺ cytotoxic T lymphocytes, thereby orchestrating immune surveillance against intracellular pathogens, tumors, and transplanted tissues. Polymorphic residues concentrated in the α1 and α2 domains create an extraordinary diversity (thousands of alleles catalogued in the IPD-IMGT/HLA database), which underpins population-level resistance to infection yet constitutes the primary barrier to solid-organ and hematopoietic stem-cell transplantation through allorecognition. Beyond antigen presentation, HLA-ABC interacts with NK-cell inhibitory receptors (KIR and LILRB1) to calibrate natural killer cell responses, and its surface levels are dynamically modulated by cytokines such as interferon-γ, viral immune evasion proteins (e.g., HIV Nef, CMV US2/US11), and oncogenic signaling pathways, making quantitative HLA-ABC expression a prognostic biomarker in cancer immunotherapy.

Picture

Western Blot

WB result of HLA-ABC Recombinant Rabbit mAb
Primary antibody: HLA-ABC Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: THP-1 whole cell lysate 20 µg
Lane 5: Raji whole cell lysate 20 µg
Lane 6: Ramos whole cell lysate 20 µg
Negative control: K562 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40 kDa
Observed MW: 40 kDa
This blot was developed with high sensitivity substrate

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human kidney. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-HLA-ABC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.