WB result of Histone H4 Recombinant Rabbit mAb
Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDa
Histone H4 Recombinant Rabbit mAb (S-499-253)
Histone H4 Recombinant Rabbit mAb (S-499-253)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Histone H4 |
| Synonyms | Histone H4; H4/A; H4FA; HIST1H4A; H4C2; H4/I; H4FI; HIST1H4B; H4C3; H4/G; H4FG; HIST1H4C; H4C4; H4/B; H4FB; HIST1H4D; H4C5; H4/J; H4FJ; HIST1H4E; H4C1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P62805 |
| Clone Number | S-499-253 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rb, Rt, Zf, Dg, Ck |
| Positive Sample | HeLa, Jurkat, NIH/3T3, mouse brain, C6, rat brain, zebrafish, dog liver, rabbit liver, pig heart, chicken liver |
| Predicted Reactivity | Fu, Dr, Pl, Ys |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Zf, Dg, Rb, Ck, Pg |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
Histone H4 is a core component of the nucleosome, the basic structural unit of chromatin in eukaryotic cells, consisting of 102 amino acids that are highly conserved across species and subject to extensive post-translational modifications—such as acetylation, methylation, phosphorylation, and ubiquitination—that collectively regulate chromatin compaction, DNA replication, repair, and transcriptional activity by altering the electrostatic interaction between DNA and the histone octamer or by recruiting specific chromatin-binding proteins.
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Picture
Western Blot
WB result of Histone H4 Recombinant Rabbit mAb
Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDa
WB result of Histone H4 Recombinant Rabbit mAb
Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDa
WB result of Histone H4 Recombinant Rabbit mAb
Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: zebrafish lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDa
WB result of Histone H4 Recombinant Rabbit mAb
Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: dog liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDa
WB result of Histone H4 Recombinant Rabbit mAb
Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rabbit liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDa
WB result of Histone H4 Recombinant Rabbit mAb
Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: chicken liver lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDa
WB result of Histone H4 Recombinant Rabbit mAb
Primary antibody: Histone H4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: pig heart lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 14 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat brian. Anti-Histone H4 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-Histone H4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-Histone H4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
